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JRC-IHCP - Poster: 'OECD validation study on the transferability, intra- and inter-laboratory reproducibility of the Zebrafish Embryo Toxicity Test (ZFET)'

News details
May 27, 2011
F. Busquet, M. Halders (JRC-IHCP) et alii

Co-authored by JRC-IHCP scientists

This poster, co-authored by scientists François Busquet and Marlies Halder (JRC-IHCP, Institute for Health and Consumer Protection) was provided as a contribution to the SETAC 2011 Annual Meeting in Milan.

Some introductory remarks and conclusions are provided below:

"One of the most promising alternative approaches to the 96h LC50 acute fish toxicity test (OECD TG 203) is based on the use of zebrafish embryos.

In fall 2005, the German Federal Environment Agency (UBA) submitted the draft test guideline “Fish embryo toxicity (FET) test” to the OECD Test Guideline Program together with a Background Paper. OECD established the ad hoc Expert Group on the Fish Embryo Toxicity Test and based on the outcome of expert meetings, OECD decided to perform a validation study (coordinated by the JRC-IHCP, European Centre for Validation of Alternative Methods (ECVAM) and steered by a validation management group). The validation study involves several international laboratories in order to evaluate the transferability of the zebrafish embryo toxicity test (ZFET) and assess its intra- and inter-laboratory reproducibility."

As a conclusion of the ZFET Phase 1:
"• ZFET was successfully transferred to the participating laboratories
• Differences in lethality observed at 48h and 96h for very toxic chemicals (1.5-2x)
• Very good intra- and inter-laboratory reproducibility of results for 4 chemicals; lower reproducibility results for 2 chemicals needs further investigation
• Analytical measurements confirm exposed test concentrations ≥ 80% except for 2 chemicals (Dibutyl maleate and 6-Methyl-5-hepten-2-one)"

The authors would like to thank the participating laboratories (University of Heidelberg, P&G, IVM, RIVM, VITO, UFZ, Ipo-Pszczyna) and the ad hoc OECD FET expert group for their contributions.