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Launch of the peer-review of the Zebrafish Embryo Toxicity Test (ZFET) for Acute Aquatic Toxicity Testing

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Oct 03, 2012
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On 27 September 2012 EURL ECVAM launched the peer review of the Zebrafish Embryo Toxicity Test (ZFET) for Acute Aquatic Toxicity Testing, to be conducted by the ECVAM Scientific Advisory Committee (ESAC)

The ZFET test method is designed to determine acute lethal effects of chemicals (including industrial chemicals, pharmaceuticals, biocides, pesticides, biocides, inorganic chemicals, etc) on pre-larval stages of the zebrafish. Newly fertilised zebrafish eggs are exposed for 96h to chemicals, lethal effects are recorded and used for calculating the concentration lethal to 50% of the embryos (LC50 value). The test is considered as a potential alternative to the Fish Acute Toxicity Test (OECD Test Guideline 203).

The peer review covers the prospective ZFET validation study on the intra- and inter-laboratory reproducibility and transferability of the ZFET (OECD validation study coordinated by EURL ECVAM) and the retrospective analysis of fish embryo toxicity and acute fish toxicity data to assess the predictive capacity of the ZFET carried out by Procter&Gamble.

A dedicated ESAC working group has been set up, which is now going to start the assessment of the reliability and relevance of the ZFET Test and to convene in a meeting on 20-21 November 2012.

Photos: Zebrafish Embryo Developmental Stages. Photos provided by J. Bachmann, UBA, Berlin. Source: IHCP poster, 2011.