The ZFET test method is designed to determine acute lethal
effects of chemicals (including industrial chemicals, pharmaceuticals,
biocides, pesticides, biocides, inorganic chemicals, etc) on pre-larval stages
of the zebrafish. Newly fertilised zebrafish eggs are exposed for 96h to
chemicals, lethal effects are recorded and used for calculating the
concentration lethal to 50% of the embryos (LC50 value). The test is considered
as a potential alternative to the Fish Acute Toxicity Test (OECD Test Guideline
The peer review covers the prospective ZFET validation study
on the intra- and inter-laboratory reproducibility and transferability of the
ZFET (OECD validation study coordinated by EURL ECVAM) and the retrospective
analysis of fish embryo toxicity and acute fish toxicity data to assess the
predictive capacity of the ZFET carried out by Procter&Gamble.
A dedicated ESAC working group has been set up, which is now
going to start the assessment of the reliability and relevance of the ZFET Test
and to convene in a meeting on 20-21 November 2012.
Photos: Zebrafish Embryo Developmental Stages. Photos provided by J. Bachmann, UBA, Berlin. Source: IHCP poster, 2011.