EURL ECVAM is inviting public comments on its recommendation on the 3T3 Neutral Red Uptake cytotoxicity assay
|Dec 19, 2012|
|Contact: JRC-IHCP, Alternatives to Animal Testing|
Deadline for comments: 31 January 2013
The European Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) is inviting public comments on its latest recommendation concerning the 3T3 Neutral Red Uptake cytotoxicity test method.
The assay was recently validated by EURL ECVAM for its possible use to support the identification of substances not requiring classification for acute oral toxicity.
The present recommendation outlines the assay's principle, its performance characteristics (based on available information and, mainly, evidence generated during validation) and the assay's limitations and makes recommendations for its implementation within an Integrated Testing Strategy (ITS) based primarily on non-animal approaches including structure-activity relationships and biokinetic modelling.
The recommendation is based on the opinion of the ECVAM EURL Scientific Advisory Committee (ESAC) which peer-reviewed the validation study (the ESAC opinion is annexed to the recommendation document).
Comments on the document and particularly on the recommendations made should be forwarded to JRC-ECVAM-CONTACT@ec.europa.eu no later than 31 January 2013.
Download document for commenting:
- Draft EURL ECVAM Recommendation of 17.12.2012 on the 3T3 Neutral Red Uptake (3T3 NRU) Cytotoxicity Assay for the identification of substances not requiring classification for Acute Oral Toxicity according to the EU Classification, Labelling and Packaging System (CLP)
Download supporting background documentation:
- ESAC Working Group Peer Review Consensus Report on the ECVAM-coordinated follow-up study to assess the predictive capacity of the already validated Neutral Red Uptake cytotoxicity assay for acute oral toxicity testing
Follow-up study on the predictive capacity of the 3T3 Neutral Red Uptake cytotoxicity assay to correctly identify substances not classified for acute oral toxicity under the EU CLP system (LD50 > 2 000 mg/kg) - Final Study Report prepared by the European Centre for the Validation of Alternative Methods (ECVAM)
Photo: Cytotoxicity/cell function-based in vitro assay. Copyright EU 2010.